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small molecular inhibitor iwp-2  (Tocris)


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    Tocris small molecular inhibitor iwp-2
    Purified human monocytes and THP1 cells were pretreated with different Wnt3a <t>inhibitors</t> (100nM IWP-2 or 1μM PNU-74654) for 2 hours or transfected with pre-validated siRNA targeting Wnt3a or Dvl3 for 48 hours, then stimulated with E. coil LPS (1μg/ml). After 24 hours stimulation, the cell-free supernatants were collected and the production of IL-12P40, IL-6, and TNFα by monocytes (A) and THP1 (B) was measured by ELISA. (C) Silencing of wnt3a or dvl3 gene in human monocytes robustly reduces the expression of Wnt3a and Dvl3, but didn’t affect other proteins (Wnt7 or Dvl2). D and E, the production of IL-12P40, IL-6, and TNFα in LPS stimulated human monocytes with or without the pre-treatment of Wnt3a siRNA (C) or Dvl3 siRNA (E). The mRNA levels of inflammatory cytokines were measured by qRT-PCR in Dvl3 siRNA-treated monocytes after 3 hours stimulation with LPS (F). *, and *** indicates statistically significant at P<0.05, and P<0.001, respectively. Data represent the arithmetic mean±S.D. of three independent experiments.
    Small Molecular Inhibitor Iwp 2, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small molecular inhibitor iwp-2/product/Tocris
    Average 90 stars, based on 1 article reviews
    small molecular inhibitor iwp-2 - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "TLR4 induced Wnt3a-Dvl3 restrains the intensity of inflammation and protects against endotoxin-driven organ failure through GSK3β/β-catenin signaling"

    Article Title: TLR4 induced Wnt3a-Dvl3 restrains the intensity of inflammation and protects against endotoxin-driven organ failure through GSK3β/β-catenin signaling

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2019.12.013

    Purified human monocytes and THP1 cells were pretreated with different Wnt3a inhibitors (100nM IWP-2 or 1μM PNU-74654) for 2 hours or transfected with pre-validated siRNA targeting Wnt3a or Dvl3 for 48 hours, then stimulated with E. coil LPS (1μg/ml). After 24 hours stimulation, the cell-free supernatants were collected and the production of IL-12P40, IL-6, and TNFα by monocytes (A) and THP1 (B) was measured by ELISA. (C) Silencing of wnt3a or dvl3 gene in human monocytes robustly reduces the expression of Wnt3a and Dvl3, but didn’t affect other proteins (Wnt7 or Dvl2). D and E, the production of IL-12P40, IL-6, and TNFα in LPS stimulated human monocytes with or without the pre-treatment of Wnt3a siRNA (C) or Dvl3 siRNA (E). The mRNA levels of inflammatory cytokines were measured by qRT-PCR in Dvl3 siRNA-treated monocytes after 3 hours stimulation with LPS (F). *, and *** indicates statistically significant at P<0.05, and P<0.001, respectively. Data represent the arithmetic mean±S.D. of three independent experiments.
    Figure Legend Snippet: Purified human monocytes and THP1 cells were pretreated with different Wnt3a inhibitors (100nM IWP-2 or 1μM PNU-74654) for 2 hours or transfected with pre-validated siRNA targeting Wnt3a or Dvl3 for 48 hours, then stimulated with E. coil LPS (1μg/ml). After 24 hours stimulation, the cell-free supernatants were collected and the production of IL-12P40, IL-6, and TNFα by monocytes (A) and THP1 (B) was measured by ELISA. (C) Silencing of wnt3a or dvl3 gene in human monocytes robustly reduces the expression of Wnt3a and Dvl3, but didn’t affect other proteins (Wnt7 or Dvl2). D and E, the production of IL-12P40, IL-6, and TNFα in LPS stimulated human monocytes with or without the pre-treatment of Wnt3a siRNA (C) or Dvl3 siRNA (E). The mRNA levels of inflammatory cytokines were measured by qRT-PCR in Dvl3 siRNA-treated monocytes after 3 hours stimulation with LPS (F). *, and *** indicates statistically significant at P<0.05, and P<0.001, respectively. Data represent the arithmetic mean±S.D. of three independent experiments.

    Techniques Used: Purification, Transfection, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR



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    small molecular inhibitor iwp-2  (Tocris)
    90
    Tocris small molecular inhibitor iwp-2
    Purified human monocytes and THP1 cells were pretreated with different Wnt3a <t>inhibitors</t> (100nM IWP-2 or 1μM PNU-74654) for 2 hours or transfected with pre-validated siRNA targeting Wnt3a or Dvl3 for 48 hours, then stimulated with E. coil LPS (1μg/ml). After 24 hours stimulation, the cell-free supernatants were collected and the production of IL-12P40, IL-6, and TNFα by monocytes (A) and THP1 (B) was measured by ELISA. (C) Silencing of wnt3a or dvl3 gene in human monocytes robustly reduces the expression of Wnt3a and Dvl3, but didn’t affect other proteins (Wnt7 or Dvl2). D and E, the production of IL-12P40, IL-6, and TNFα in LPS stimulated human monocytes with or without the pre-treatment of Wnt3a siRNA (C) or Dvl3 siRNA (E). The mRNA levels of inflammatory cytokines were measured by qRT-PCR in Dvl3 siRNA-treated monocytes after 3 hours stimulation with LPS (F). *, and *** indicates statistically significant at P<0.05, and P<0.001, respectively. Data represent the arithmetic mean±S.D. of three independent experiments.
    Small Molecular Inhibitor Iwp 2, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small molecular inhibitor iwp-2/product/Tocris
    Average 90 stars, based on 1 article reviews
    small molecular inhibitor iwp-2 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    Purified human monocytes and THP1 cells were pretreated with different Wnt3a inhibitors (100nM IWP-2 or 1μM PNU-74654) for 2 hours or transfected with pre-validated siRNA targeting Wnt3a or Dvl3 for 48 hours, then stimulated with E. coil LPS (1μg/ml). After 24 hours stimulation, the cell-free supernatants were collected and the production of IL-12P40, IL-6, and TNFα by monocytes (A) and THP1 (B) was measured by ELISA. (C) Silencing of wnt3a or dvl3 gene in human monocytes robustly reduces the expression of Wnt3a and Dvl3, but didn’t affect other proteins (Wnt7 or Dvl2). D and E, the production of IL-12P40, IL-6, and TNFα in LPS stimulated human monocytes with or without the pre-treatment of Wnt3a siRNA (C) or Dvl3 siRNA (E). The mRNA levels of inflammatory cytokines were measured by qRT-PCR in Dvl3 siRNA-treated monocytes after 3 hours stimulation with LPS (F). *, and *** indicates statistically significant at P<0.05, and P<0.001, respectively. Data represent the arithmetic mean±S.D. of three independent experiments.

    Journal: Molecular immunology

    Article Title: TLR4 induced Wnt3a-Dvl3 restrains the intensity of inflammation and protects against endotoxin-driven organ failure through GSK3β/β-catenin signaling

    doi: 10.1016/j.molimm.2019.12.013

    Figure Lengend Snippet: Purified human monocytes and THP1 cells were pretreated with different Wnt3a inhibitors (100nM IWP-2 or 1μM PNU-74654) for 2 hours or transfected with pre-validated siRNA targeting Wnt3a or Dvl3 for 48 hours, then stimulated with E. coil LPS (1μg/ml). After 24 hours stimulation, the cell-free supernatants were collected and the production of IL-12P40, IL-6, and TNFα by monocytes (A) and THP1 (B) was measured by ELISA. (C) Silencing of wnt3a or dvl3 gene in human monocytes robustly reduces the expression of Wnt3a and Dvl3, but didn’t affect other proteins (Wnt7 or Dvl2). D and E, the production of IL-12P40, IL-6, and TNFα in LPS stimulated human monocytes with or without the pre-treatment of Wnt3a siRNA (C) or Dvl3 siRNA (E). The mRNA levels of inflammatory cytokines were measured by qRT-PCR in Dvl3 siRNA-treated monocytes after 3 hours stimulation with LPS (F). *, and *** indicates statistically significant at P<0.05, and P<0.001, respectively. Data represent the arithmetic mean±S.D. of three independent experiments.

    Article Snippet: The small molecular inhibitors, N -(6-methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2- d ] pyrimidin-2-yl) thio]-acetamide (IWP-2) and benzoic acid, 2-phenoxy-,2-[(5-methyl-2-furanyl) methylene] hydrazide (PNU-74654), were from Tocris Biosciences (Minneapolis, MN).

    Techniques: Purification, Transfection, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR